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human cd8 t cell isolation kit  (Miltenyi Biotec)


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    Structured Review

    Miltenyi Biotec human cd8 t cell isolation kit
    Human Cd8 T Cell Isolation Kit, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 97/100, based on 379 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cd8+t+cells/CD8%2B+T+Cell+Isolation+Kit%2C+human/10__1007_slash_s00262___026___04553___2-47-5-11
    Average 97 stars, based on 379 article reviews
    human cd8 t cell isolation kit - by Bioz Stars, 2026-10
    97/100 stars

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    Related Articles

    Purification:

    Article Title: ATR inhibition in combination with hypofractionated radiotherapy is superior to ATM inhibition with regard to ex vivo CD8 + T cell activation particularly for HPV-negative head and neck cancer cells.
    Article Snippet: Briefly, peripheral blood mononuclear cells (PBMCs) were first obtained from EDTA-anticoagulated whole blood by density gradient centrifugation using Ficoll-Paque. .. CD8+ T cells were subsequently purified by positive selection using magnetically labeled microbeads (CD8 MicroBeads human, Cat# 130–045-201, Miltenyi Biotec B.V. & Co. KG, Bergisch Gladbach, Germany) according to the manufacturer’s protocol (140–000-034.08). ..

    Selection:

    Article Title: ATR inhibition in combination with hypofractionated radiotherapy is superior to ATM inhibition with regard to ex vivo CD8 + T cell activation particularly for HPV-negative head and neck cancer cells.
    Article Snippet: Briefly, peripheral blood mononuclear cells (PBMCs) were first obtained from EDTA-anticoagulated whole blood by density gradient centrifugation using Ficoll-Paque. .. CD8+ T cells were subsequently purified by positive selection using magnetically labeled microbeads (CD8 MicroBeads human, Cat# 130–045-201, Miltenyi Biotec B.V. & Co. KG, Bergisch Gladbach, Germany) according to the manufacturer’s protocol (140–000-034.08). ..

    Article Title: Upregulation of TNFR2 Precedes TOX Expression by Exhausted T cells and Restricts Antitumor and Antiviral Immunity
    Article Snippet: Enrichment was achieved using the “possel” program on the Miltenyi autoMACS. .. In spleens from cLCMV-infected mice, a negative selection for CD8 T cells (Miltenyi Biotec, cat. #130-104-075) was performed according to the manufacturer’s protocol. .. Enrichment was achieved using the “deplete” program on the Miltenyi autoMACS.

    Labeling:

    Article Title: ATR inhibition in combination with hypofractionated radiotherapy is superior to ATM inhibition with regard to ex vivo CD8 + T cell activation particularly for HPV-negative head and neck cancer cells.
    Article Snippet: Briefly, peripheral blood mononuclear cells (PBMCs) were first obtained from EDTA-anticoagulated whole blood by density gradient centrifugation using Ficoll-Paque. .. CD8+ T cells were subsequently purified by positive selection using magnetically labeled microbeads (CD8 MicroBeads human, Cat# 130–045-201, Miltenyi Biotec B.V. & Co. KG, Bergisch Gladbach, Germany) according to the manufacturer’s protocol (140–000-034.08). ..

    Isolation:

    Article Title: High-risk EBV promotes immune evasion in nasopharyngeal carcinoma by upregulating HLA-DP via the encoded BALF2-HR variant.
    Article Snippet: .. Primary CD4+T cells (Miltenyi Biotec, 130-045-101, Germany) and CD8+T cells (Miltenyi Biotec, 130-045-201, Germany) were isolated from PBMCs using magnetic separation with CD4/CD8 microbeads. .. Primary CD4+T cells (Miltenyi Biotec, 130-045-101, Germany) and CD8+T cells (Miltenyi Biotec, 130-045-201, Germany) were isolated from PBMCs using magnetic separation with CD4/CD8 microbeads.

    Article Title: Pharmacologic DPP-4 inhibition promotes CD8⁺ T cell metabolic fitness to enhance anti-tumor activity
    Article Snippet: .. Frozen stocks were then thawed, and CD8 + T cells were isolated using human CD8 MicroBeads (Miltenyi #130-045-201). ..

    other:

    Article Title: High-risk EBV promotes immune evasion in nasopharyngeal carcinoma by upregulating HLA-DP via the encoded BALF2-HR variant
    Article Snippet: Protein bands were visualized using ECL detection reagent (Coolaber, SL1350, China), and images were captured with the Mini Chemiluminescent Imaging and Analysis System.

    Incubation:

    Article Title: Effect of Oral Ketone Body Intake on Human CD8 + T-Cell Immunometabolism
    Article Snippet: .. CD8 + T cells were incubated with the respective antibody for 30 min on ice in the dark (CD25 Antibody, anti-human, Vio ® Bright B515, 130-113-849; CD38 Antibody, anti-human, APC, 130-113-991; CD44 Antibody, anti-human, PE, 130-113-904; CD69 Antibody, anti-human, FITC, 130-112-801; all Miltenyi Biotec, Bergisch-Gladbach, Germany). .. Flow cytometric acquisition was performed using a BD FACSCanto II (BD Biosciences, San Jose, CA, USA), and data were analyzed with FlowJo v10 (FlowJo, Ashland, OR, USA).

    FACS:

    Article Title: T cells compete via reverse MHC class I signaling at the synapse with dendritic cells to secure Golgi recruitment for activation
    Article Snippet: .. CD8 + T cells were obtained after LD column magnetic antibody cell sorting (MACS, 130-042-901, Miltenyi Biotec) with anti-CD8 antibody beads from Miltenyi Biotec (130-104-075). ..

    Magnetic Cell Separation:

    Article Title: T cells compete via reverse MHC class I signaling at the synapse with dendritic cells to secure Golgi recruitment for activation
    Article Snippet: .. CD8 + T cells were obtained after LD column magnetic antibody cell sorting (MACS, 130-042-901, Miltenyi Biotec) with anti-CD8 antibody beads from Miltenyi Biotec (130-104-075). ..



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    Overexpression of UPF1 in ccRCC inhibits M2 macrophage polarization and enhances the tumor suppressor function of CD8 + T cells, Caki-1 cell groups of sh-NC+PBS, sh-UPF1+PBS, or sh-UPF1 + 2-DG, and the conditioned medium was used to culture and polarize M0 macrophages. The polarized macrophages were cultured with activated CD8 + T cells. (A) CFSE assessed CD8 + T cell proliferative capacity. (B) Flow cytometry measured apoptosis rate of CD8 + T cells in different co-culture systems. (C) CD8 + T cells from culture systems were co-cultured with normal Caki-1 cells, with LDH assays determining cytotoxic effect of CD8 + T cells. *, p < 0.05. cRCC, Clear cell renal cell carcinoma; UPF1, Up-frameshift protein 1; 2-DG, 2-Deoxy-D-glucose; CFSE, Carboxyfluorescein succinimidyl ester.

    Journal: Frontiers in Immunology

    Article Title: UPF1-dependent glycolysis driven by LINC00887 facilitates macrophage M2 polarization in clear cell renal cell carcinoma

    doi: 10.3389/fimmu.2026.1833383

    Figure Lengend Snippet: Overexpression of UPF1 in ccRCC inhibits M2 macrophage polarization and enhances the tumor suppressor function of CD8 + T cells, Caki-1 cell groups of sh-NC+PBS, sh-UPF1+PBS, or sh-UPF1 + 2-DG, and the conditioned medium was used to culture and polarize M0 macrophages. The polarized macrophages were cultured with activated CD8 + T cells. (A) CFSE assessed CD8 + T cell proliferative capacity. (B) Flow cytometry measured apoptosis rate of CD8 + T cells in different co-culture systems. (C) CD8 + T cells from culture systems were co-cultured with normal Caki-1 cells, with LDH assays determining cytotoxic effect of CD8 + T cells. *, p < 0.05. cRCC, Clear cell renal cell carcinoma; UPF1, Up-frameshift protein 1; 2-DG, 2-Deoxy-D-glucose; CFSE, Carboxyfluorescein succinimidyl ester.

    Article Snippet: Primary CD8 + Cytotoxic T Cells (PCS-800-017) were from ATCC (USA).

    Techniques: Over Expression, Cell Culture, Flow Cytometry, Co-culture Assay

    LINC00887/UPF1-mediated aerobic glycolysis leads to lactic acid accumulation and facilitates immune evasion in ccRCC in vivo , A syngeneic tumor model was developed by injecting BALB/c mice with Renca cells expressing sh-NC or sh-LINC00887, followed by treatment with sh-NC or sh-UPF1 lentivirus. A-C: Assessment of tumor size (A) , volume (B) , and weight (C) . (D, E) qRT-PCR analyses for LINC00887 (D) and UPF1 (E) mRNA levels. (F) IHC analysis for UPF1, LDH-A, KI67 protein levels. (G) Kit assay for lactate levels in tumors. (H) Proportion of M2 macrophages (F4/80+CD206+) by flow cytometry. (I) Immunofluorescence assay for CD206+ macrophages. (J) Proportion of CD8 + T cells (CD3 + CD8 + ) in tumor tissues. ns, no significant difference. * p < 0.05. cRCC, Clear cell renal cell carcinoma; UPF1, Up-frameshift protein 1; LDH-A, Lactate dehydrogenase A; CD206, Mannose receptor C-type 1; CD3, Cluster of differentiation 3; CD8, Cluster of differentiation 8.

    Journal: Frontiers in Immunology

    Article Title: UPF1-dependent glycolysis driven by LINC00887 facilitates macrophage M2 polarization in clear cell renal cell carcinoma

    doi: 10.3389/fimmu.2026.1833383

    Figure Lengend Snippet: LINC00887/UPF1-mediated aerobic glycolysis leads to lactic acid accumulation and facilitates immune evasion in ccRCC in vivo , A syngeneic tumor model was developed by injecting BALB/c mice with Renca cells expressing sh-NC or sh-LINC00887, followed by treatment with sh-NC or sh-UPF1 lentivirus. A-C: Assessment of tumor size (A) , volume (B) , and weight (C) . (D, E) qRT-PCR analyses for LINC00887 (D) and UPF1 (E) mRNA levels. (F) IHC analysis for UPF1, LDH-A, KI67 protein levels. (G) Kit assay for lactate levels in tumors. (H) Proportion of M2 macrophages (F4/80+CD206+) by flow cytometry. (I) Immunofluorescence assay for CD206+ macrophages. (J) Proportion of CD8 + T cells (CD3 + CD8 + ) in tumor tissues. ns, no significant difference. * p < 0.05. cRCC, Clear cell renal cell carcinoma; UPF1, Up-frameshift protein 1; LDH-A, Lactate dehydrogenase A; CD206, Mannose receptor C-type 1; CD3, Cluster of differentiation 3; CD8, Cluster of differentiation 8.

    Article Snippet: Primary CD8 + Cytotoxic T Cells (PCS-800-017) were from ATCC (USA).

    Techniques: In Vivo, Expressing, Quantitative RT-PCR, Analysis, Flow Cytometry, Immunofluorescence