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dead cell removal kit  (Miltenyi Biotec)


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    Structured Review

    Miltenyi Biotec dead cell removal kit
    Dead Cell Removal Kit, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 99/100, based on 2477 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cd8+t+cells/Dead+Cell+Removal+Kit/custom%40130-090-101%4042466067
    Average 99 stars, based on 2477 article reviews
    dead cell removal kit - by Bioz Stars, 2026-09
    99/100 stars

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    Purification:

    Article Title: ATR inhibition in combination with hypofractionated radiotherapy is superior to ATM inhibition with regard to ex vivo CD8 + T cell activation particularly for HPV-negative head and neck cancer cells.
    Article Snippet: Briefly, peripheral blood mononuclear cells (PBMCs) were first obtained from EDTA-anticoagulated whole blood by density gradient centrifugation using Ficoll-Paque. .. CD8+ T cells were subsequently purified by positive selection using magnetically labeled microbeads (CD8 MicroBeads human, Cat# 130–045-201, Miltenyi Biotec B.V. & Co. KG, Bergisch Gladbach, Germany) according to the manufacturer’s protocol (140–000-034.08). ..

    Selection:

    Article Title: ATR inhibition in combination with hypofractionated radiotherapy is superior to ATM inhibition with regard to ex vivo CD8 + T cell activation particularly for HPV-negative head and neck cancer cells.
    Article Snippet: Briefly, peripheral blood mononuclear cells (PBMCs) were first obtained from EDTA-anticoagulated whole blood by density gradient centrifugation using Ficoll-Paque. .. CD8+ T cells were subsequently purified by positive selection using magnetically labeled microbeads (CD8 MicroBeads human, Cat# 130–045-201, Miltenyi Biotec B.V. & Co. KG, Bergisch Gladbach, Germany) according to the manufacturer’s protocol (140–000-034.08). ..

    Article Title: Upregulation of TNFR2 Precedes TOX Expression by Exhausted T cells and Restricts Antitumor and Antiviral Immunity
    Article Snippet: Enrichment was achieved using the “possel” program on the Miltenyi autoMACS. .. In spleens from cLCMV-infected mice, a negative selection for CD8 T cells (Miltenyi Biotec, cat. #130-104-075) was performed according to the manufacturer’s protocol. .. Enrichment was achieved using the “deplete” program on the Miltenyi autoMACS.

    Labeling:

    Article Title: ATR inhibition in combination with hypofractionated radiotherapy is superior to ATM inhibition with regard to ex vivo CD8 + T cell activation particularly for HPV-negative head and neck cancer cells.
    Article Snippet: Briefly, peripheral blood mononuclear cells (PBMCs) were first obtained from EDTA-anticoagulated whole blood by density gradient centrifugation using Ficoll-Paque. .. CD8+ T cells were subsequently purified by positive selection using magnetically labeled microbeads (CD8 MicroBeads human, Cat# 130–045-201, Miltenyi Biotec B.V. & Co. KG, Bergisch Gladbach, Germany) according to the manufacturer’s protocol (140–000-034.08). ..

    Isolation:

    Article Title: High-risk EBV promotes immune evasion in nasopharyngeal carcinoma by upregulating HLA-DP via the encoded BALF2-HR variant.
    Article Snippet: .. Primary CD4+T cells (Miltenyi Biotec, 130-045-101, Germany) and CD8+T cells (Miltenyi Biotec, 130-045-201, Germany) were isolated from PBMCs using magnetic separation with CD4/CD8 microbeads. ..

    Article Title: Pharmacologic DPP-4 inhibition promotes CD8⁺ T cell metabolic fitness to enhance anti-tumor activity
    Article Snippet: .. Frozen stocks were then thawed, and CD8 + T cells were isolated using human CD8 MicroBeads (Miltenyi #130-045-201). ..

    Article Title: High-risk EBV promotes immune evasion in nasopharyngeal carcinoma by upregulating HLA-DP via the encoded BALF2-HR variant
    Article Snippet: .. Primary CD4+ T cells (Miltenyi Biotec, 130-045-101, Germany) and CD8+T cells (Miltenyi Biotec, 130-045-201, Germany) were isolated from PBMCs using magnetic separation with CD4/CD8 microbeads. ..

    Incubation:

    Article Title: Effect of Oral Ketone Body Intake on Human CD8 + T-Cell Immunometabolism
    Article Snippet: .. CD8 + T cells were incubated with the respective antibody for 30 min on ice in the dark (CD25 Antibody, anti-human, Vio ® Bright B515, 130-113-849; CD38 Antibody, anti-human, APC, 130-113-991; CD44 Antibody, anti-human, PE, 130-113-904; CD69 Antibody, anti-human, FITC, 130-112-801; all Miltenyi Biotec, Bergisch-Gladbach, Germany). .. Flow cytometric acquisition was performed using a BD FACSCanto II (BD Biosciences, San Jose, CA, USA), and data were analyzed with FlowJo v10 (FlowJo, Ashland, OR, USA).

    FACS:

    Article Title: T cells compete via reverse MHC class I signaling at the synapse with dendritic cells to secure Golgi recruitment for activation
    Article Snippet: .. CD8 + T cells were obtained after LD column magnetic antibody cell sorting (MACS, 130-042-901, Miltenyi Biotec) with anti-CD8 antibody beads from Miltenyi Biotec (130-104-075). ..

    Magnetic Cell Separation:

    Article Title: T cells compete via reverse MHC class I signaling at the synapse with dendritic cells to secure Golgi recruitment for activation
    Article Snippet: .. CD8 + T cells were obtained after LD column magnetic antibody cell sorting (MACS, 130-042-901, Miltenyi Biotec) with anti-CD8 antibody beads from Miltenyi Biotec (130-104-075). ..



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